Objective: WEE1 Kinase is associated with controlling the cell cycle progression through G2/M check point. Inhibition of this Kinase has been shown to induce apoptosis in a variety of cancerous cells by skipping the repair mechanisms. In majority of cancer cells, the G1/S check point is damaged due to p53 mutations. So inhibition of this Kinase seems to be a justified approach for induction of DNA damage mediated apoptosis with normal p53 positive cells being excluded. In this study the effect of WEE1 shRNA mediated suppression was evaluated in a cell line from Non small cell lung cancer, considering the reported high profile of WEE1 expression in NSCLC.Materials and Methods: WEE1 gene was silenced using the specific shRNA in QU-DB cell line. Transfection efficacy was examined by flow cytometery based on GFP expression.48 hours post-transfection, the fixed DNA content and permealized cells was stained with Propodium Iodide and evaluated by flow cytometery. WEE1 protein expression was also assessed by Western Blotting.Results: Analysis of Cell Cycle by flow cytometry and after PI staining showed that the majority of treated cells were within the M phase of cell cycle 48h post treatment On the other hand, Analysis of protein content in treated cells and the control group by Western blotting was indicative of successful WEE1 suppression.Conclusion: The significant progression through Mphase is suggestive of the prominent role of WEE1 in G2/M regulation. We concerned WEE1 suppression might be a good candidate to fight against NSCLC. Inhibition of cell cycle checkpoint regulators has also been exploited to improve the efficacy of stem cell and iPSCs production. Therefore, this Kinase would be a suggestion for further studies in this field.